UNIT 1
- Basic Concept and Principal of Biotechnology
- History of Biotechnology
- Scope of Biotechnology
- Plant tissue Culture General Introduction
- Basic Aspects of Plant Tissue culture
- Culture Media
- Basic nutritional composition of plant tissue culture media
- Types of culture
- Basic technique of plant tissue culture
- Concept of Cellular differentiation totipotency
- Callus Cultures
- Organogenesis
- Somatic Embryogenesis
- Androgenesis
UNIT 2
- Somatic Hybridization
- Isolation of protoplasts
- Protoplast culture techniques
- Protoplast Regeneration
- Protoplast Fusion and Somatic Hybridization
- Selection of Somatic Hybrids
- Identification of hybrid (cell) plants
- Cybrid
- Applications of Somatic Hybridization
- Limitation of Somatic Hybridization
- Clonal Propagation (Micro propagation)
- Recombinant DNA Technology Tools and Technique of rDNA Tech
- Gene Cloning Principle and Technique
- Construction of Genomic and cDNA Library
- cDNA Libraries
- DNA Fingerprinting
UNIT 3
- Genetic Engineering of Plants: Aims strategies for development of transgenic
- History of Genetic Engineering:
- Strategies of Genetic Engineering
- Agrobacterium: nature’s genetic engineer
- T DNA Transfer and integration
- Transposon Tagging
- Transposon Tagging Strategies
- Chloroplast Transformation
- Intellectual Property Right Possible ecological risks and Ethical concern
- Biosafety and Bioethics
- Microbial Genetic Manipulation -Bacterial Transformation
UNIT 4
