Limitation of Somatic Hybridization
There are several problems and limitations in somatic hybridization. some of which are listed below.
- Somatic hybridization does not always produce plants that give fertile and visible seeds.
- Regenerated plants obtained from somatic hybridization are often variable due to somaclonal variations, chromosomal elimination, organelle segregation etc.
- Protoplast culture is frequently associated with genetic instability.
- Protoplast fusion between different species/ genus is easy, but the production of viable somatic hybrids is not possible in all instances.
- Some of the somatic hybrids, particularly when produced by the fusion of taxonomically different partners are unbalanced and not viable.
- There are limitations in the selection methods of hybrids, as many of them are not efficient.
- There is no certainty as regards the expression of any specific character in somatic hybridization.
- Somatic hybridization between two diploids results in the formation of an amphidiploid which is not favourable. For this reason, haploid protoplasts are recommended in somatic hybridization.