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Basic technique of plant tissue culture

• The explant eg. bids stem, seed are trimmed and then subjected to sterilization in a detergent solution.
• After washing in sterile distilled water the explants are placed in a suitable culture medium(liquid or semisolid) and incubated .
• This results in the establishment of culture.
• The mother culture can be subdivided as frequently as needed to give daughter cultures.
• The most important aspect of in vitro culture technique is to carry out all the operations under aseptic conditions.

• Bacteria and fungi are the most common contaminants in plant tissue culture .
• They grow much faster in culture and often kill the plant tissue.
• Further the contaminants also produce certain compounds which are toxic to the plant tissue .
• Therefore it is absolutely essential that aseptic conditions are maintained throughout the tissue culture operations.