Basic technique of plant tissue culture
- The explant eg. bids stem, seed are trimmed and then subjected to sterilization in a detergent solution.
- After washing in sterile distilled water the explants are placed in a suitable culture medium(liquid or semisolid) and incubated .
- This results in the establishment of culture.
- The mother culture can be subdivided as frequently as needed to give daughter cultures.
- The most important aspect of in vitro culture technique is to carry out all the operations under aseptic conditions.
- Bacteria and fungi are the most common contaminants in plant tissue culture .
- They grow much faster in culture and often kill the plant tissue.
- Further the contaminants also produce certain compounds which are toxic to the plant tissue .
- Therefore it is absolutely essential that aseptic conditions are maintained throughout the tissue culture operations.