Mitochondria (Power house of cell )

Mito – thread chondrion – granule

  • These are rod shaped structures,
  • function as power generators of cell as they convert nutrient and oxygen in to ATP
  • It is chemical energy which is essential to carry out the metabolic process of cell.

History-

  • 1880 – Koliker – discover in Insects muscles cells and give name – Sarcosome 
  • 1882 – Flaming – Thread like structure
  • 1884 – Altman – Stain – Janus Green (Stain use in Lab), Alizarin +C.V. Give name – Bioplast.
  • 1898 – Benda – Give name – Mitochondria
  • 1904 – Discover in Plant (Nymphea) by – Meves
  • 1953 – Palade and Porter – Study Electron Microscope
  • 1963- Nass – Discover mtDNA

Presence and Distribution

  • It is present in all eukaryotic cells except RBC and Prokaryotic Cell
  • These are freely distributed in cytoplasm
  • These are found in large number in which cell where much more energy are required

Number – 

In Metabolically active cell – 1 Lakh or more / Cell

Size –

  • Generally it is – 0.5 μ wide and 1.5 – 10μ long 
  • Smallest – Yeast – 1mμ
  • It can also 40μ long

Shape – 

Cylindrical , Rod, Filamentous, Round  Granular, Clubshape, Vesicular.

Basic Structure & Function of Mitochondria

1. What is the primary function of mitochondria?

  • A) Protein synthesis
  • B) Energy production
  • C) DNA replication
  • D) Lipid synthesis
  • Answer: B

2. Mitochondria generate energy in the form of which molecule?

  • A) DNA
  • B) RNA
  • C) ATP
  • D) Glucose
  • Answer: C

3. Which of the following is NOT a characteristic of mitochondria?

  • A) Rod-shaped
  • B) Have their own DNA
  • C) Present in RBCs
  • D) Double-membrane structure
  • Answer: C

4. The term “Mitochondria” was first coined by:

  • A) Koliker
  • B) Benda
  • C) Nass
  • D) Palade
  • Answer: B

5. Mitochondria are absent in:

  • A) Liver cells
  • B) Muscle cells
  • C) Red blood cells
  • D) Nerve cells
  • Answer: C

Discovery & History of Mitochondria

6. Who first discovered mitochondria in insect muscle cells?

  • A) Nass
  • B) Koliker
  • C) Altman
  • D) Benda
  • Answer: B

7. Who gave the name ‘Sarcosome’ to mitochondria?

  • A) Koliker
  • B) Benda
  • C) Flaming
  • D) Meves
  • Answer: A

8. Who first observed mitochondria as thread-like structures?

  • A) Benda
  • B) Flaming
  • C) Altman
  • D) Koliker
  • Answer: B

9. What stain was used by Altman to study mitochondria?

  • A) Safranin
  • B) Janus Green
  • C) Methylene Blue
  • D) Eosin
  • Answer: B

10. Mitochondria were discovered in plant cells (Nymphea) by:

  • A) Meves
  • B) Koliker
  • C) Benda
  • D) Porter
  • Answer: A

Structure & Components of Mitochondria

11. How many membranes do mitochondria have?

  • A) One
  • B) Two
  • C) Three
  • D) None
  • Answer: B

12. What is the inner membrane of mitochondria called?

  • A) Cristae
  • B) Thylakoid
  • C) Stroma
  • D) Matrix
  • Answer: A

13. The mitochondrial matrix contains:

  • A) Ribosomes
  • B) Enzymes
  • C) DNA
  • D) All of the above
  • Answer: D

14. The space between the inner and outer mitochondrial membranes is called:

  • A) Intermembrane space
  • B) Stroma
  • C) Thylakoid space
  • D) Periplasmic space
  • Answer: A

15. The inner membrane of mitochondria is folded into:

  • A) Vesicles
  • B) Granules
  • C) Cristae
  • D) Filaments
  • Answer: C

Mitochondrial DNA & Function

16. Who discovered mitochondrial DNA (mtDNA)?

  • A) Altman
  • B) Nass
  • C) Koliker
  • D) Meves
  • Answer: B

17. Mitochondrial DNA is inherited from:

  • A) Father
  • B) Mother
  • C) Both parents
  • D) Grandparents
  • Answer: B

18. The number of mitochondria in a cell depends on:

  • A) The cell’s energy requirement
  • B) The cell’s size
  • C) The cell’s shape
  • D) Random distribution
  • Answer: A

19. Mitochondrial DNA is:

  • A) Linear
  • B) Circular
  • C) Double-stranded
  • D) Both B and C
  • Answer: D

20. The major function of cristae in mitochondria is to:

  • A) Store ATP
  • B) Increase surface area for ATP production
  • C) Store oxygen
  • D) Synthesize proteins
  • Answer: B

Metabolism & Energy Production

21. Which metabolic process occurs in mitochondria?

  • A) Glycolysis
  • B) Krebs Cycle
  • C) Photosynthesis
  • D) Fermentation
  • Answer: B

22. What is the end product of mitochondrial respiration?

  • A) Oxygen
  • B) ATP
  • C) Water
  • D) Carbon dioxide
  • Answer: B

23. The electron transport chain occurs in which part of mitochondria?

  • A) Cristae
  • B) Matrix
  • C) Outer membrane
  • D) Cytoplasm
  • Answer: A

24. The powerhouse of the cell is called so because it:

  • A) Stores ATP
  • B) Generates ATP
  • C) Transports nutrients
  • D) Breaks down proteins
  • Answer: B

25. The enzyme ATP synthase is located in:

  • A) Inner mitochondrial membrane
  • B) Outer mitochondrial membrane
  • C) Matrix
  • D) Ribosomes
  • Answer: A

Miscellaneous Facts & Advanced Topics

26. Which shape is NOT associated with mitochondria?

  • A) Rod-shaped
  • B) Club-shaped
  • C) Spherical
  • D) Star-shaped
  • Answer: D

27. Which type of cells have the highest number of mitochondria?

  • A) Nerve cells
  • B) Skin cells
  • C) Muscle cells
  • D) Bone cells
  • Answer: C

28. The smallest mitochondria are found in:

  • A) Yeast
  • B) Bacteria
  • C) Algae
  • D) Fungi
  • Answer: A

29. Which of the following is NOT a feature of mitochondria?

  • A) Self-replicating
  • B) Circular DNA
  • C) Ribosomes
  • D) Cell wall
  • Answer: D

30. Which molecule acts as the final electron acceptor in the mitochondrial electron transport chain?

  • A) ATP
  • B) Oxygen
  • C) Carbon dioxide
  • D) NADH
  • Answer: B

Advanced Topics in Mitochondria

31. What is the primary role of mitochondria in apoptosis (programmed cell death)?

  • A) DNA replication
  • B) Energy storage
  • C) Release of cytochrome c
  • D) Protein synthesis
  • Answer: C

32. What is the role of cytochrome c in mitochondria?

  • A) ATP synthesis
  • B) Electron transport chain
  • C) Cell division
  • D) Lipid breakdown
  • Answer: B

33. Which of the following mitochondrial enzymes is responsible for ATP production?

  • A) DNA polymerase
  • B) ATP synthase
  • C) RNA polymerase
  • D) Catalase
  • Answer: B

34. Which of the following mitochondrial functions is crucial for cell survival?

  • A) Glycolysis
  • B) Photosynthesis
  • C) Oxidative phosphorylation
  • D) Fermentation
  • Answer: C

35. Mitochondrial dysfunction is linked to which of the following diseases?

  • A) Diabetes
  • B) Alzheimer’s
  • C) Parkinson’s
  • D) All of the above
  • Answer: D

36. Which hypothesis explains the origin of mitochondria?

  • A) Cell Theory
  • B) Endosymbiotic Theory
  • C) Spontaneous Generation
  • D) Germ Theory
  • Answer: B

37. Which evidence supports the endosymbiotic theory of mitochondrial origin?

  • A) Presence of circular DNA
  • B) 70S ribosomes similar to bacteria
  • C) Ability to self-replicate
  • D) All of the above
  • Answer: D

38. The TCA (Krebs) cycle occurs in which part of mitochondria?

  • A) Cristae
  • B) Matrix
  • C) Outer membrane
  • D) Intermembrane space
  • Answer: B

39. Mitochondria use which molecules as fuel for ATP production?

  • A) Proteins
  • B) Lipids
  • C) Carbohydrates
  • D) All of the above
  • Answer: D

40. The mitochondria in sperm cells are located in:

  • A) The head
  • B) The tail
  • C) The midpiece
  • D) The acrosome
  • Answer: C

Mitochondrial Inheritance & Evolution

41. Which type of inheritance is followed by mitochondrial DNA?

  • A) Maternal
  • B) Paternal
  • C) Both maternal and paternal
  • D) Random inheritance
  • Answer: A

42. Which type of ribosomes are present in mitochondria?

  • A) 80S
  • B) 60S
  • C) 70S
  • D) 90S
  • Answer: C

43. What is the role of mitochondria in thermogenesis (heat production)?

  • A) ATP synthesis
  • B) Uncoupling protein (UCP) activation
  • C) Oxygen storage
  • D) Water production
  • Answer: B

44. Which type of specialized fat cells contain many mitochondria for heat generation?

  • A) White adipose tissue
  • B) Brown adipose tissue
  • C) Yellow adipose tissue
  • D) Red adipose tissue
  • Answer: B

45. Which process occurs when mitochondria generate ATP without oxygen?

  • A) Oxidative phosphorylation
  • B) Anaerobic respiration
  • C) Glycolysis
  • D) Fermentation
  • Answer: B

46. Mitochondria have a protein-import system called:

  • A) TOM and TIM complexes
  • B) SOS system
  • C) ABC transporters
  • D) Endocytosis
  • Answer: A

47. The presence of mitochondrial diseases can be diagnosed by:

  • A) Muscle biopsy
  • B) DNA sequencing
  • C) Enzyme activity tests
  • D) All of the above
  • Answer: D

48. Which organelle works closely with mitochondria in lipid metabolism?

  • A) Ribosome
  • B) Endoplasmic reticulum
  • C) Golgi apparatus
  • D) Lysosome
  • Answer: B

49. Which mitochondrial disorder is caused by mutations in mtDNA?

  • A) Leigh syndrome
  • B) Alzheimer’s disease
  • C) Parkinson’s disease
  • D) Cystic fibrosis
  • Answer: A

50. In which part of mitochondria is oxygen consumed?

  • A) Outer membrane
  • B) Intermembrane space
  • C) Matrix
  • D) Cristae
  • Answer: D

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