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MOLECULAR ORGANISATION OF CENTROMERE AND TELOMERE

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MOLECULAR ORGANISATION OF CENTROMERE AND TELOMERE Centromere (i) Conserved element I (CDE I): (ii) Conserved element II (CDE II): (iii) Conserved element III (CDE III): Telomere 🔹 PART A: CENTROMERE STRUCTURE & FUNCTION Q1. What is the primary function of the centromere in a chromosome?A. RNA synthesisB. DNA replication✅ C. Attachment site for spindle fibersD. … Read more

Nucleosome

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Nucleosome Chromatin    → Nucleosomal monomers Containing the spacer DNA (160 base pairs)    →    Core nucleosomes (140 base pairs) →     Subnucleosomal particles 🔹 PART A: Basic Concept of Nucleosome Q1. The term “nucleosome” was first coined by:A. Olius and Woodcock✅ B. Oudet et al.C. Watson and CrickD. Hershey and Chase Q2. … Read more

CHROMOSOME STRUCTURE

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CHROMOSOME STRUCTURE Detailed structure of chromosome Chromatid 1- Sister chromatid 2-Non sister chromatid Chromonema –  Centromere/Primary constriction – According to the no. of centromere these are the following types According to the position of centromere chromosomes are following types 1. Telocentric – 2. Acrocentric 3. Sub metacentric  4. Metacentric 4. Kinetochore – 5. Chromomere – … Read more

CHROMOSOMAL ORGANISATION

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CHROMOSOMAL ORGANISATION (Chroma-color, soma-body) Chromosome Numbers At the time of gametes formation, their number remains half (N), that becomes 2N again after fertilization. Eukaryotic Chromosome Organization The following material are present in the Eukaryotic Chromosome 1.DNA RNA Protein Two types of proteins are present in chromosomes. 1- Histone protein 2- Non – Histone protein 3- … Read more

Confocal Microscopy

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Confocal Microscopy Types Basic Concepts – Imaging Modes –  Three-Color Imaging for Confocal Microscopy –  True or False: Scanning electron microscopes and laser scanning confocal microscopes both can produce 3D images.– Answer: True– UK OCR A‑Level Biology, June 2020 OpenStax+7Scribd+7OCR+7OCR+1Study Mind+1 **Which microscope captures optical sections for 3D reconstructions?**a) Phase contrastb) Differential interference contrastc) Confocal … Read more

GISH

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GISH The Main steps involved in the genomic in situ hybridization are:  The major application of GISH technique are as follows: 

FISH-Fluorescent in situ hybridization

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FISH-Fluorescent in situ hybridization Preparation and Hybridization Process Variations on Probes and Analysis Medical applications Species identification

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